분획으로 분리한 Streptococcus mutans KCTC 3065 다당류의 화학적 특성
Chemical properties of Streptococcus mutans KCTC 3065 polysaccharide purified by fractions
소속 상세정보
한만덕/Han, Man-Deuk
이준우/나수정/이은숙/전은숙/Lee, June-Woo/Ra, Su-Jung/Lee, Eun-Sook/Jeon, Eun-Suk
KMID : 0355420000240030259
Abstract
The oral micro-organism Streptococcus mutans synthesizes extracellular glucosyltransferase(GTFs) which polymerize the glucose moiety of sucrose into glucan polymers. These glucan was known to cause dental caries. The study was set up to elucidate the chemical properties of polysaccharides obtained from the cell wall(CWP) and extracellular polysaccharides(ECP) of S. mutans KCTC 3065 polysaccharides. The recovery rate(%) of extracellular and cell wall polysaccharides were purified of 0.032% and 0.13% of total polysaccharide(dry weight), respectively.
CWP fraction was determined to be approximately average 70 kilodalton by gel filtration, contains 62,2% of D-glucose and 38.9% of galactose. Whereas, ECP appears approximately average 60 kD by gel filtration, and contains exclusively a-D-glucose, fructose and mannose.
키워드
Streptococcus mutans;세포외다당류;세포벽다당류;글루칸;분리;특성
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